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Molecular Biology

DNA, RNA, protein concentration, PCR, OD260, cell culture & bioinformatics calculators

7 Calculators
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DNA Concentration (OD260)

Calculate DNA concentration from absorbance at 260 nm. 1 OD260 = 50 μg/mL for double-stranded DNA.

Molecular Biology

DNA Copy Number Calculator

Convert DNA mass (ng) to number of molecules (copy number) for a given template length.

Molecular Biology

Melting Temperature (Tm)

Calculate DNA melting temperature (Tm) for primers and oligos using the nearest-neighbor method (simplified).

Molecular Biology

PCR Amplification Calculator

Calculate how much DNA you'll get after N PCR cycles. Each cycle doubles the DNA.

Molecular Biology

Protein Concentration (from OD280)

Estimate protein concentration from absorbance at 280 nm using the extinction coefficient.

Molecular Biology

Cell Seeding Density

Calculate how many cells to seed and what volume of suspension to add to a culture vessel.

Molecular Biology

Molarity ↔ Mass (any MW)

Convert between molarity and mass for any substance using its molecular weight.

Molecular Biology
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